土壤质量 污染物对蚯蚓的影响 第2部分:对蚯蚓繁殖的影响的测定

[中文适用范围]： 本文件规定了评估土壤栖息地功能并确定土壤污染物和化学物质对赤子爱胜蚓/安氏爱胜蚓通过皮肤和食物吸收繁殖的影响的方法之一。 该长期测试适用于质量未知的土壤和土壤材料，例如来自污染场地、改良土壤、修复后的土壤、农业或其他相关场地以及废料。 该方法主要用于测定土壤污染物和化学物质对赤子爱胜蚓/安氏爱胜蚓繁殖的影响。 还提供了如何使用 Eisenia fetida/andrei 在热带条件下测试化学品的技术信息（见附件 A）。 最后，该方法还包括如何将其与其他环境相关的蚯蚓物种一起使用的技术信息：例如 Dendrodrilus rubidus 和 Aporectodea caliginosa（见附件 B 和 C）。 本方法不适用于空气/土壤分配系数大于1的物质，或25℃时蒸气压超过300Pa的物质。 该方法没有考虑测试过程中物质的持久性。 [外文原描述]： This document specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. This method is designed mainly for determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei . Technical information is also provided on how to use Eisenia fetida/andrei for testing chemicals under tropical conditions (see Annex A ). Finally, this method also includes technical information on how to use it with other environmentally relevant earthworm species: e.g. Dendrodrilus rubidus and Aporrectodea caliginosa (see Annexes B and C ). This method does not apply to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa, at 25 °C. This method does not take into account the persistence of the substance during the test.