标准摘要
[中文适用范围]: 本国际标准规定了一种使用高效液相色谱(HPLC)和光散射检测器,通过二醇柱定量测定磷脂含量的方法。该方法适用于植物油来源的含油粗卵磷脂、无油卵磷脂及卵磷脂组分。该方法不适用于动物和反刍动物卵磷脂以及酶解卵磷脂,因为溶磷脂酰乙醇胺(LPE)、溶磷脂酰肌醇(LPI)和溶磷脂酸(LPA)的峰分离不足。 [外文原描述]: ISO 11701:2009 specifies a method for the quantitative determination of phospholipids content by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector. The method is applicable to crude, oil-containing lecithins, and to oil-free lecithins and lecithin fractions from vegetable fats and oils. The method is not applicable to animal and ruminant lecithins and enzymatically hydrolysed lecithins as the peak separation of lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient.
英文名称Vegetable fats and oils — Determination of phospholipids content in lecithins by HPLC using a light-scattering detector