动物和植物的脂肪和油.总甾醇含量的酶催化测定

[中文适用范围]： 本标准规定了用酶染色法定量测定总甾醇含量的方法。 本方法适用于动植物油脂、脂肪食品及相关产品中的游离和酯化甾醇。 该测定适用于1g至2g脂肪的样品量。 该方法不适用于深色脂肪和油。 该酶并非专门针对胆固醇，但也会氧化其他 3-羟基甾醇。 该方法尚未针对高浓度甾醇强化产品进行测试。 奶和奶制品（或来自奶和奶制品的脂肪）不属于本国际标准的范围。 注：该方法在技术上等同于 IUPAC 方法 2.404[8] 和 DGF 标准方法 F-III 2 (91)。 [7] [外文原描述]： ISO 11702:2016 specifies a method for the quantitative determination of the total sterols content by means of an enzymatic staining test. The method is applicable to free and esterified sterols in animal and vegetable fats and oils, fatty foods and related products. The determination is applicable to sample quantities of 1 g to 2 g of fat. The method is not applicable to dark coloured fats and oils. The enzyme is not specific for cholesterol, but also oxidizes other 3-hydroxysterols. The method has not been tested for products fortified with sterols at higher levels. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of ISO 11702:2016.