水质.水介质中有机化合物的生物降解和去除率的测定.活性污泥模拟试验

[中文适用范围]： 本标准规定了好氧微生物对有机化合物消除和生物降解能力的测定方法。 所描述的条件模拟废水处理厂。 可以使用两种测试系统：活性污泥装置或多孔罐。 测试可以选择在硝化和反硝化（附件 A）以及单元耦合（附件 B）的条件下进行。 该方法适用于在测试条件下的有机化合物： a) 在测试浓度下可溶于自来水，并且如果确定除消除外还进行生物降解，预计不会转化为不溶性代谢物； b) 水溶性差，但在水中的分散性令人满意，并允许用合适的分析方法进行检测（例如有机碳测量）； c) 不挥发，或在测试条件下具有可忽略不计的蒸气压； d) 在测试所选择的浓度下对测试微生物没有抑制作用。 抑制效果可以通过使用合适的测试方法（例如ISO 8192 或ISO 15522t）来确定。 可以在低于其EC20值的浓度下测试本测试中所用浓度的抑制性化合物，然后在适应一段时间后测试更高的实际浓度。 该方法还可用于测量废水中溶解的有机化合物（在该方法中也称为“测试化合物”）的生物降解和消除。 如果需要更多或不同的信息来预测处理厂中测试化合物或废水的行为，则可以进行其他降解测试。 有关该方法的正确使用和替代生物降解方法，请参阅 ISO/TR 15462，有关生物测试的一般信息，请参阅 ISO 5667-16。 [外文原描述]： ISO 11733:2004 specifies a method for the determination of the elimination and the biodegradability of organic compounds by aerobic micro-organisms. The conditions described simulate a wastewater treatment plant. Two test systems can be used: activated sludge plants or porous pots. The tests can optionally be performed under conditions of nitrification and denitrification and coupling of the units. The method applies to organic compounds which, under the conditions of the test, are soluble in tap water at the test concentration and not expected to be transformed to insoluble metabolites if biodegradation, in addition to elimination, is determined; poorly water-soluble, but which are satisfactorily dispersible in water and allow detection with suitable analytical means (e.g. organic carbon measurements); non-volatile, or which have a negligible vapour pressure under the test conditions; not inhibitory to the test micro-organisms at the concentration chosen for the test. Compounds inhibitory at concentrations used in this test may be tested at concentrations less than their EC 20 value, followed by higher practical concentrations after a period of acclimatization. The method can also be used to measure the biodegradation and elimination of dissolved organic compounds in waste water (also called "test compound" in the method).