标准摘要
[中文适用范围]: ISO 11866⎪IDF 170 的这一部分规定了通过涉及 MUG 液体培养基的培养技术来计数推定大肠杆菌和推定大肠菌群的组合方法,并计算每个推定大肠杆菌和/或大肠菌群的数量。 30°C 孵育后,通过最可能数 (MPN) 技术计算克或每毫升。 这是一种比 ISO 7251 中描述的方法更快速的方法,因为缩短了孵育时间(省略了几个富集步骤)。 本方法适用于 -- 牛奶、液态奶制品, -- 奶粉、甜乳清粉、酪乳粉、乳糖, -- 酸性酪蛋白、乳酸酪蛋白和凝乳酶酪蛋白, -- 酪蛋白酸盐和干酸乳清, -- 奶酪和加工奶酪, -- 黄油, -- 冷冻奶制品(包括食用冰),以及 -- 蛋奶冻、甜点和奶油。 ISO 11866⎪IDF 170 本部分规定的方法适用于疑似推定大肠杆菌和/或其他推定大肠菌群数量相对较低(每克少于 100 个或每毫升少于 10 个)的样品。 [外文原描述]: ISO 11866-1|IDF 170-1:2005 specifies a combined method for the enumeration of presumptive Escherichia coli and of presumptive coliforms by means of a culture technique involving a liquid medium with MUG, and calculation of the number of presumptive Escherichia coli and/or coliforms per gram or per millilitre by the most probable number (MPN) technique after incubation at 30 °C. The method is applicable to milk, liquid milk products, dried milk, dried sweet whey, dried buttermilk, lactose, acid casein, lactic casein and rennet casein, caseinate and dried acid whey, cheese and processed cheese, butter, frozen milk products (including edible ices), and custard, desserts and cream. The method specified in ISO 11866-1|IDF 170-1:2005 is preferred for samples in which comparatively low numbers of presumptive Escherichia coli and/or other presumptive coliforms (less than 100 per gram or 10 per millilitre) are suspected.
英文名称Milk and milk products — Enumeration of presumptive Escherichia coli — Part 1: Most probable number technique using 4-methylumbelliferyl-beta-D-glucuronide (MUG)