动物和植物油脂和脂肪 脂肪酸甲酯的测定 第4部分：毛细管气相色谱法（ISO/DIS 12966-4:2025）

[中文适用范围]： 本文件规定采用毛细管气相色谱法（GLC）测定由脂肪、油脂及脂肪酸经酯交换或酯化反应生成的脂肪酸甲酯（FAMEs）的方法。本文件适用于分离C4至C24的FAMEs，包括饱和FAMEs、顺式及反式单不饱和FAMEs，以及顺式及反式多不饱和FAMEs。本文件适用于来源于动物和植物的未精制、精制、部分氢化或完全氢化的脂肪、油脂及脂肪酸，以及从食品中提取的脂肪。本文件不适用于乳及乳制品（包括乳及乳制品中的脂肪），也不适用于添加共轭亚油酸（CLA）的制品。本文件不适用于双酯、三酯、聚合物、羟基化和氧化脂肪酸及其对应的脂肪与油脂。附录E提出了以面积百分比表示的液体植物油中FAMEs组成的测定方法。 [外文原描述]： This document specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). FAMEs from C4 to C24 can be separated using this document including saturated FAMEs, cis - and trans -monounsaturated FAMEs, and cis - and trans -polyunsaturated FAMEs. This document is applicable to crude, refined, partially hydrogenated or fully hydrogenated fats, oils and fatty acids derived from animal and vegetable sources, and fats extracted from foodstuff. This document does not apply to milk and milk products (or fat coming from milk and milk products) or products supplemented with conjugated linoleic acid (CLA). This document does not apply to di-, tri-, polymerized, hydroxylated and oxidized fatty acids, and fats and oils. A method for the determination of the composition of FAMEs expressed by area % in liquid vegetable oils is proposed in Annex E .