肉和肉制品.着色剂的检测和测定

[中文适用范围]： 本标准规定了肉和肉制品中合成着色剂的薄层色谱检测方法和高效液相色谱（HPLC）测定方法。 本文件指定 HPLC 方法作为参考方法。 本文件适用于肉类及肉制品，包括畜禽产品。 使用薄层色谱的方法可以检测以下着色剂： ——柠檬黄 — 专利蓝 V — 喹啉黄 — 靛蓝 — 日落黄 FCF — 亮黑 PN — 苋菜红 — 黑色 7984 — 丽春红 4R — 固绿 FCF — 赤藓红 — 蓝色 VRS这些着色剂的同义词和识别号列于附录 A。 已观察到不干扰本方法的植物色素和植物提取物列于 B.1。 B.2 中列出了在某些情况下会干扰此方法的自然颜色。 使用 HPLC 的方法可以检测以下着色剂： ——柠檬黄 — 诱惑红 AC — 苋菜红 — 亮蓝 FCF — 丽春红 4R — 新红 — 日落黄 FCF — 胭脂红 — 赤藓红 — 靛蓝 这些标准参考颜色的色谱图见附录 D 。 [外文原描述]： This document specifies a detection method using thin-layer chromatography and a determination method using high performance liquid chromatography (HPLC) for synthetic colouring agents in meat and meat products. This document specifies the HPLC method as the reference method. This document is applicable to meat and meat products, including livestock and poultry products. The method using thin-layer chromatography can detect the following colouring agents: Tartrazine, Patent Blue V, Quinoline Yellow, Indigotine, Sunset Yellow FCF, Brilliant Black PN, Amaranth, Black 7984, Ponceau 4R, Fast Green FCF, Erythrosine, Blue VRS. Synonyms and identity numbers of these colouring agents are listed in Annex A. The plant colours and plant extracts which have been observed not to interfere with this method are listed in B.1. Natural colours which in some cases have been shown to interfere with this method are listed in B.2. The method using HPLC can detect the following colouring agents: Tartrazine, Allura Red AC, Amaranth, Brilliant Blue FCF, Ponceau 4R, New Red, Sunset Yellow FCF, Carmoisine, Erythrosine, Indigotine. Chromatograms of these standard reference colours are shown in Annex D.