标准摘要
[中文适用范围]： ISO 13641的这一部分规定了一种筛选方法，通过确定泥浆、沉积物中沼气（二氧化碳和甲烷）的产生来评估物质、混合物、地表水、地下水和废水、废水、污泥或其他环境样品的潜在毒性。 以及其他生物量浓度低的厌氧环境。 与需氧微生物相比，厌氧细菌的生长速度要低得多。 因此，厌氧方法的测试时间比需氧细菌的测试时间长。 该测试的条件（例如测试瓶中的接种量和底物的量）适用于数天的规定测试周期。 接种物可以从厌氧沉积物或大型或实验室规模的厌氧消化池中收集。 该方法适用于可溶于水或不溶于水的材料，包括挥发性化学物质（参见参考文献中的参考文献[1]）。 注：对于低水溶性化合物，需要特别小心，在这些情况下，请参见 ISO 10634。 有关生物测试的一般信息，请参见 ISO 5667-16。 通过该方法获得的信息有助于在低接种物质量浓度的厌氧生物降解性测试之前以及估计化学品和废水对厌氧生物量相对较低的栖息地（例如天然沉积物和土壤）中厌氧过程的潜在影响。 [外文原描述]： ISO 13641-2:2003 specifies a screening method for assessing the potential toxicity of substances, mixtures, surface waters, groundwaters and wastewaters, effluents, sludges, or other environmental samples by determining the production of biogas (carbon dioxide and methane) from muds, sediments and other anaerobic environments with low biomass concentration. The growth rate of anaerobic bacteria is much lower, compared with that of aerobic microorganisms. For this reason, the test periods in anaerobic methods are longer than in those with aerobic bacteria. The conditions of this test (for example amount of inoculum and substrate in the test bottles) were adopted to a defined test period over several days. The inoculum can be collected from anaerobic sediments or from large, or laboratory scale, anaerobic digesters. This method is applicable to materials, soluble or insoluble in water, including volatile chemicals. Note that special care is necessary with compounds of low water-solubility, and in these cases, other methods might be more suitable. Information obtained by this method may be helpful prior to anaerobic biodegradability testing with low inoculum mass concentrations and for estimations of the potential effects of chemicals and wastewater to anaerobic processes in habitats characterized by a relatively low anaerobic biomass, for example natural sediments and soils.
英文名称Water quality — Determination of inhibition of gas production of anaerobic bacteria — Part 2: Test for low biomass concentrations