标准摘要
[中文适用范围]: 本标准规定了乳和奶粉中黄曲霉毒素M1(AFM1)含量的测定方法,该方法包括使用免疫亲和色谱净化步骤和薄层色谱(IAC-TLC)步骤。 本方法适用于生乳、低脂或脱脂液态奶和奶粉。 通常可测定的 AFM1 最低量为 2 ng,这相当于液态奶或溶解奶粉的定量限接近 0.10 μg/l(对于 20 μl 点)。 [外文原描述]: ISO 14674|IDF 190:2005 specifies a method for the determination of the aflatoxin M 1 (AFM1) content of milk and milk powder by a method including a clean-up step using immunoaffinity chromatography followed by a thin-layer chromatography (IAC-TLC). The method is applicable to raw milk, low fat or skimmed liquid milk and milk powder. The lowest quantity of AFM1 that can commonly be determined is 2 ng, which corresponds to a limit of quantification close to 0,10 micrograms per litre for liquid milk or dissolved milk powder (for a spot of 20 microlitres).
英文名称Milk and milk powder — Determination of aflatoxin M1 content — Clean-up by immunoaffinity chromatography and determination by thin-layer chromatography