食物链微生物学.梭状芽孢杆菌检测和计数的水平方法.第1部分：用菌落计数技术计数减少亚硫酸盐的梭状芽孢菌

[中文适用范围]： 本文件规定了亚硫酸盐还原梭菌属的计数。 通过菌落计数技术。 本文件适用于： ——供人类消费的产品； ——饲养动物的产品； ——食品和饲料生产和处理领域的环境样品； ——初级生产阶段的样品。 注：该方法已在以下食品类别的实验室间研究中得到验证： ——即食、即热肉制品； ——鸡蛋和蛋制品（衍生物）； ——加工过的水果和蔬菜； ——婴儿配方奶粉和婴儿谷物食品； ——多成分食物或膳食成分。 它还已针对以下其他类别进行了验证： ——宠物食品和动物饲料； ——环境样品（食品或饲料生产）。 由于该方法已针对至少五类食品进行了验证，因此该方法适用于广泛的食品。 有关验证的详细信息，请参阅第 11 条和附录 C。 由于该方法不常用于初级生产阶段的样品，因此该类别未包含在实验室间研究中。 因此，没有获得该类别的性能特征。 这种水平方法最初是为了检查属于食物链的所有样品而开发的。 根据本文件发布时可获得的信息，该方法被认为完全适合检查属于食物链的所有样品。 然而，由于食物链中的产品种类繁多，这种水平方法可能并不适合所有产品的每个细节。 然而，预计所需的修改将被最小化，以便它们不会导致与该水平方法的显着偏差。 该技术适用于但不限于对测试样品中的微生物进行计数，并在平板上计数至少 10 个菌落。 这对应于液体样品预计高于 10 cfu/ml 或固体样品高于 100 cfu/g 的污染水平。 [外文原描述]： This document specifies the enumeration of sulfite-reducing Clostridium spp. by the colony-count technique. This document is applicable to: — products intended for human consumption; — products for feeding animals; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. NOTE This method has been validated in an interlaboratory study for the following food categories: — ready-to-eat, ready-to-reheat meat products; — eggs and egg products (derivates); — processed fruits and vegetables; — infant formula and infant cereals; — multi-component foods or meal components. It has also been validated for the following other categories: — pet food and animal feed; — environmental samples (food or feed production). As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C . Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.