食物链微生物学梭菌属检测和计数的水平方法第2部分:菌落计数技术对产气荚膜梭菌的计数

[中文适用范围]： 本文件规定了用菌落计数法对产气荚膜梭菌 (C.) 进行计数。本文件适用于： — 供人类食用的产品； — 动物饲养产品； — 食品和饲料生产和处理领域的环境样品； — 初级生产阶段的样品。 注： 该方法已在实验室间研究中针对以下食品类别进行了验证： — 即食、即热肉制品； — 蛋类和蛋制品（衍生物）； — 加工水果和蔬菜； — 婴儿配方奶粉和婴儿谷类食品； — 多组分食品或膳食组分。该方法还针对以下其他类别进行了验证： — 宠物食品和动物饲料； — 环境样品（食品或饲料生产）。 由于该方法已针对至少五种食品类别进行了验证，因此该方法适用于广泛的食品。有关验证的详细信息，请参阅第 11 条和附件 C。由于该方法通常不用于初级生产阶段的样品，因此该类别未包括在实验室间研究中。因此，没有获得此类别的性能特征。这种水平方法最初是为检查属于食品链的所有样品而开发的。根据本文发布时可用的信息，该方法被认为完全适合检查属于食品链的所有样品。但是，由于食品链中的产品种类繁多，这种水平方法可能并不适用于所有产品的每一个细节。尽管如此，预计所需的修改将最小化，以便它们不会导致与这种水平方法的显着偏差。该技术适用于（但不限于）对测试样品中的微生物进行计数，每皿上至少计数 10 个菌落。这相当于液体样品的污染水平预计高于 10 cfu/ml，固体样品的污染水平预计高于 100 cfu/g。 [外文原描述]： This document specifies the enumeration of Clostridium (C.) perfringens by colony-count technique. This document is applicable to: — products intended for human consumption; — products for feeding animals; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. NOTE This method has been validated in an interlaboratory study for the following food categories: — ready-to-eat, ready-to-reheat meat products; — eggs and egg products (derivates); — processed fruits and vegetables; — infant formula and infant cereals; — multi-component foods or meal components. It has also been validated for the following other categories: — pet food and animal feed; — environmental samples (food or feed production). As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C . Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.