标准摘要
[中文适用范围]： 本文件规定了一种测定软水果、绿叶菜、块茎类蔬菜、瓶装水和BMS样品（或食品表面）中HAV和诺罗病毒I型(GI)和II型(GII)RNA含量的方法。经过释放测试样品中的病毒后，通过裂解液胍的异硫氰酸盐吸附到二氧化硅上来提取病毒RNA。通过实时RT-PCR对目标序列进行扩增和检测。此方法未在其他食物（包括多表面或其他基质成分）或任何其他基质中验证用于检测靶病毒，也不用于检测食物中的其他病毒 [外文原描述]： ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.
英文名称Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 1: Method for quantification