水质 水体组分对活性污泥中微生物增长抑制影响的测定

[中文适用范围]： 本国际标准规定了评估测试材料对活性污泥中好氧细菌生长的潜在毒性的方法。 抑制作用仅限于那些能够在所选有机测试培养基上生长的微生物。 该方法提供了在长达 6 小时的潜伏期内对微生物的抑制作用的信息。 本方法适用于在试验条件下可溶解的水、废水和化学物质。 需要特别注意挥发性或有色材料以及形成混浊悬浮液或分散体的材料。 注 1：应谨慎解释挥发性测试材料的结果，并且由于难以维持测试烧瓶中的初始浓度，因此可能会低估任何抑制作用。 注 2：在许多情况下，可以使用颜色/浊度对照作为空白来测试形成混浊悬浮液或分散体的有色材料和低水溶性材料（见 9.4）。 注 3：本试验不适用的试验材料对活性污泥微生物的抑制作用可以通过使用基于呼吸测量的抑制试验来确定（见 ISO 8192）。 [外文原描述]： This International Standard specifies a method for assessing the potential toxicity of a test material to the growth of aerobic bacteria present in activated sludge. The inhibitory effect is restricted to those microorganisms capable of growth on the chosen organic test medium. This method gives information on inhibitory effects on the microorganisms over incubation periods up to 6 h. This method is applicable to water, wastewater and chemical substances which are soluble under the conditions of the test. Special care is needed with volatile or coloured materials and materials which form turbid suspensions or dispersions. NOTE 1 Results with volatile test material should be interpreted with caution and are likely to underestimate any inhibitory effect because of the difficulties in maintaining the initial concentration in the test flasks. NOTE 2 Coloured materials and materials of low water-solubility which form turbid suspensions or dispersions can be tested in many cases using colour/turbidity controls as blanks (see 9.4). NOTE 3 Inhibitory effects on activated sludge microorganisms of test materials for which this test is not applicable can be determined by using an inhibition test based on respirometric measurements (see ISO 8192).