化妆品.微生物学.酵母与霉菌的计数

[中文适用范围]： 本文件提供了通过在有氧培养后对选择性琼脂培养基上的菌落进行计数来计数化妆品中存在的酵母和霉菌的一般指南。 为了确保消费者的产品质量和安全，建议进行适当的微生物风险分析，以确定本文件适用的化妆品类型。 被认为具有低微生物风险的产品（参见 ISO 29621）包括具有低水分活度或极端 pH 值的产品、水醇产品等。 由于该应用领域内的化妆品种类繁多，因此该方法可能不适用适合某些产品的各个细节（例如某些与水不混溶的产品）。 其他方法（例如自动方法）可以替代此处介绍的测试，只要它们的等效性已得到证明或该方法已被证明是合适的。 可以使用合适的鉴定测试来鉴定所计数的酵母，例如参考文献中列出的标准中描述的测试。 如有必要，可以通过其他适当的方法来识别所列举的霉菌。 [外文原描述]： ISO 16212:2017 gives general guidelines for enumeration of yeast and mould present in cosmetics by counting the colonies on selective agar medium after aerobic incubation. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which ISO 16212:2017 is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity or extreme pH values, hydro-alcoholic products, etc. Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other methods (e.g. automated) can be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable. Yeast enumerated can be identified using suitable identification tests, for example, tests described in the standards listed in the Bibliography. Mould enumerated can be identified by other appropriate methods, if necessary.