标准摘要
[中文适用范围]： 本文件规定了定量聚合酶链式反应（qPCR）方法的关键步骤，用于测定从土壤DNA提取物中选出的微生物基因序列的丰度。通过qPCR量化出的微生物基因序列数量，可估计选定微生物群在土壤中的丰度。 [外文原描述]： This document specifies the crucial steps of a quantitative polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract. The number of microbial gene sequences quantified by qPCR provides an estimation of the abundance of selected microbial groups in soil.
英文名称Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative polymerase chain reaction (qPCR) from DNA directly extracted from soil