水质 水体环境DNA的采样、捕获与保存

[中文适用范围]： 1 适用范围 本文件规定了在水生环境中采集、保存源自生物体（包括当前存在于水体中或近期曾存在于水体中的生物体，或其DNA通过某种机制被引入水体中的生物体）的环境DNA（eDNA）的操作程序。本文件还规定了在过滤和保存过程中防止样本污染并确保eDNA完整性的程序，并明确了所需设备及元数据报告要求。 本文件不适用于以下情况： ——从生物膜、沉积物或类似样本类型中采集eDNA的方法； ——被动采样方法； ——采样设计方案。 [外文原描述]： This document specifies procedures for sampling, capture and preservation of environmental DNA (eDNA) in aquatic environments, originating from organisms that are present or have recently been present in a water body, or whose DNA has been introduced to the water body through some mechanism. This document also specifies procedures for avoiding sample contamination and ensuring the integrity of environmental DNA during water filtration and sample preservation. It also specifies the required equipment and metadata reporting. This document excludes: methods for the collection of eDNA from biofilms, sediments or similar sample types; passive sampling methods; sampling designs.