水质 - 耐热弯曲杆菌属物种的检测和计数

[中文适用范围]： 本文件规定了耐热弯曲杆菌属物种的检测、半定量和定量（MPN）计数方法。 该方法可应用于各种水体，包括：饮用水、地下水和井水、淡水、咸水和咸水地表水、游泳池、水疗和水疗池、娱乐用水、农业用水和径流、未经处理和处理过的废水和还有沙子和其他沉积物。 该方法可用于检测指定样品体积中的弯曲杆菌属。 低浊度的清洁水样可采用膜过滤进行定性、半定量或定量 (MPN) 方法。 通过将材料直接接种到瓶子或试管中，使用相同的定性、半定量或定量 MPN 方法对浊度较高的水样（例如初级和二级废水流出物和沉积物）进行分析。 沉淀物可以悬浮在合适的稀释剂中或直接接种到增菌肉汤中。 希望使用此方法的用户应在自己的实验室条件下验证其针对特定基质的性能。 [外文原描述]： This document specifies a method for the detection, semi-quantitative and quantitative (MPN) enumeration of thermotolerant Campylobacter species. The method can be applied to all kinds of waters including: drinking water, ground water and well water, fresh, brackish and saline surface water, swimming pools, spa and hydrotherapy pools, recreational waters, agricultural waters and runoff, untreated and treated wastewater and also sand and other sediments. This method can be used for the detection of Campylobacter species in a specified sample volume. Clean water samples with low turbidity can be membrane filtered for either a qualitative method, semi-quantitative or quantitative (MPN) method. Water samples with higher turbidity, such as primary and secondary wastewater effluents and sediments, are analysed using the same qualitative, semi-quantitative or quantitative MPN method by direct inoculation of material into bottles or tubes. Sediments can be suspended in a suitable diluent or inoculated directly into enrichment broths. Users wishing to employ this method are expected to verify its performance for the particular matrix under their own laboratory conditions.