标准摘要
[中文适用范围]： 本文件规定了在油和脂肪中以结合态或游离态存在的甘油醇（glycidol）及其2-环氧化物（2-MCPD）和3-环氧化物（3-MCPD）的同时测定程序。该方法基于碱性催化的酯裂解、释放的甘油醇转化为单溴丙二醇（MBPD）以及游离二醇（MCPD 和 MBPD）与苯基硼onic 酸（PBA）的衍生物化。尽管游离 MCPD 和 glycidol 在脂肪和油中的含量较低或可忽略不计，但如果存在游离分析物，则会按比例贡献结果。结果始终是单个分析物的自由和结合形式的总和。该方法适用于固体和液体脂肪和油。本文件也可应用于动物脂肪和使用过的煎炸油和脂肪，但在分析这些基质之前需要进行验证研究。牛奶及其制品（或来自牛奶及其制品的脂肪）不在本文件的范围内。 [外文原描述]： This document specifies a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MCPD present in bound or free form in oils and fats. The method is based on alkaline-catalysed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derivatisation of the derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). Though free MCPD and glycidol are supposed to be present in fats and oils in low to negligible quantities only, in the event that free analytes are present, they would contribute proportionately to the results. The results always being the sum of the free and the bound form of a single analyte. This method is applicable to solid and liquid fats and oils. This document can also apply to animal fats and used frying oils and fats, but a validation study is undertaken before the analysis of these matrices. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
英文名称Animal and vegetable fats and oils — Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS — Part 2: Method using slow alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol