标准摘要
[中文适用范围]： 本文件规定了基于碱性催化酯裂解，在单次测定中同时测定 2-MCPD 酯（结合 2-MCPD）、3-MCPD 酯（结合 3-MCPD）和缩水甘油酯（结合缩水甘油）的快速程序在 GC-MS/MS 分析之前，用苯基硼酸 (PBA) 对裂解的（游离）分析物进行衍生化。 通过添加同位素标记的酯结合的 3-MCPD 来纠正缩水甘油酯的过高估计，这允许在操作过程中对 3-MCPD 诱导的缩水甘油进行定量。 该方法适用于固体和液体油脂。 本文件也适用于动物脂肪和使用过的煎炸油和脂肪，但这些基质未包括在验证中。 对于所有三种分析物，定量限 (LOQ) 为 0.1 mg/kg，检测限 (LOD) 为 0.03 mg/kg。 奶和奶制品（或来自奶和奶制品的脂肪）、婴儿配方奶粉、乳化剂、游离脂肪酸和其他脂肪和油衍生的基质不包括在本文件的范围内。 [外文原描述]： This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure. This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg. Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.
英文名称Animal and vegetable fats and oils — Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS — Part 4: Method using fast alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol by GC-MS/MS