动植物脂肪和油.用高效粒度排除色谱法(HPSEC)测定单酰甘油、二酰甘油、三酰甘油和丙三醇

[中文适用范围]： 本标准规定了高效尺寸排阻色谱法测定单酰基甘油、二酰基甘油和三酰基甘油以及游离甘油的方法。 它适用于以单酰基甘油和二酰基甘油为主要成分且浓度>10%，以及三酰基甘油含量<20%的产品（例如乳化剂）。 该方法不适用于乳脂肪或具有宽范围脂肪酸链长度的脂肪和油，因为短链脂肪酸的二酰基甘油具有比长链脂肪酸的单酰基甘油更低的分子量。 该方法对基于辛酸和癸酸的酰基甘油混合物的适用性受到限制。 在此，只能测定单酰基甘油含量和游离甘油含量。 注：参考文献 [1] 至 [4] 提供了背景信息。 [外文原描述]： ISO 18395:2005 specifies a method for the determination of monoacylglycerols, diacylglycerols and triacylglycerols and also free glycerol by high-performance size-exclusion chromatography. It is applicable to products (e.g. emulsifiers) comprising monoacylglycerols and diacylglycerols as main constituents in concentrations greater than 10 %, and to triacylglycerols in a proportion of less than 20 %. The method is not applicable to dairy fats or fats and oils having a wide range of fatty acid chain lengths, since diacylglycerols of short fatty acids have a lower molecular mass than monoacylglycerols of long-chain fatty acids. The method has restricted applicability to acylglycerol mixtures based on caprylic and capric acids. Here, only the monoacylglycerol content and the free glycerol content can be determined.