化妆品.微生物学.白色念球菌检测

[中文适用范围]： 本国际标准提供了在化妆品产品中检测和鉴定特定微生物白色念珠菌的一般指南。根据国家实践或法规，本国际标准中视为特定的微生物可能因国家而异。为了确保产品质量和消费者安全，建议进行适当的微生物风险分析，以确定本国际标准适用的化妆品类型。被认为具有低微生物风险的产品（见ISO 29621）包括那些具有低水活性、醇水产品、极端pH值等。本标准中描述的方法基于在非选择性液体培养基（富集肉汤）中检测白色念珠菌，随后在选择性琼脂培养基上分离。根据所需的检测水平，其他方法可能适用。 [外文原描述]： ISO 18416:2015 gives general guidelines for the detection and identification of the specified microorganism Candida albicans in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc. The method described in this International Standard is based on the detection of Candida albicans in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate dependent on the level of detection required. NOTE For the detection of Candida albicans, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits). Because of the large variety of cosmetic products within this field of application, this method may not be appropriate in every detail for some products (e.g. certain water immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.