食物链的微生物学. 使用人工消化法检测肉中的旋毛虫幼虫

[中文适用范围]： 本国际标准规定了旋毛虫属的检测方法。 供人类食用的个体动物尸体肉中的肌肉期幼虫。 适用于可感染旋毛虫属线虫的家畜和森林动物肉类的检验。 该方法无法鉴定检测到的寄生虫的种类或基因型；物种或基因型鉴定可以通过分子方法进行。 本国际标准中描述的方法旨在与世界动物卫生组织诊断测试和疫苗手册以及国际旋毛虫病委员会 (ICT) 中的指南结合使用，用于旋毛虫测试和供人类食用的屠体检查，除非已通过其他方式证明该动物没有接触旋毛虫的风险。 人工消化/磁力搅拌器方法被认为是标准方法，因为它已被证明可以在验证研究中给出最可靠的结果。 注：如果可以记录与本国际标准中描述的方法的等效性，则可以使用替代方法进行分析。 [外文原描述]： ISO 18743:2015 specifies a method of detection of Trichinella spp. muscle stage larvae in meat of individual animal carcasses intended for human consumption. It is applicable to the examination of meat from domestic and sylvatic animal species, which can be infected by nematodes of the genus Trichinella . This method does not allow the identification of the species or genotype of detected parasites; species or genotype identification can be carried out by molecular methods. The method described in this International Standard is intended to be used in conjunction with the guidelines in the OIE Manual of Diagnostic Tests and Vaccines and by the International Commission on Trichinellosis (ICT) for Trichinella testing and the inspection of carcasses intended for human consumption, unless it has been demonstrated by other means that the animal was not at risk for exposure to Trichinella . The artificial digestion/magnetic stirrer method is considered to be the standard method because it has proven to give the most reliable results in validation studies. NOTE Provided equivalence with the method described within this International Standard can be documented, alternative methods can be used for analysis.