食物链微生物学.食品中葡萄球菌肠毒素免疫酶检测的水平方法

[中文适用范围]： 本文件规定了食品中葡萄球菌肠毒素 SEA、SEB、SEC、SED 和 SEE 的筛查方法。 它由两个主要步骤组成：a) 提取，然后根据透析原理进行浓缩； b) 使用市售检测试剂盒进行免疫酶检测。 本文件适用于人类消费产品中葡萄球菌肠毒素 SEA 至 SEE 的筛查。 其他葡萄球菌肠毒素，如 SEG、SEH、SEI、SER、SES 和 SET 型也可引起疾病。 由于缺乏市售检测试剂盒，本文件仅适用于 SEA 至 SEE 类型，但也可能适用于其他类型的毒素，但需经过方法验证。 [外文原描述]： ISO 19020:2017 specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE in foodstuffs. It consists of two main steps: a) extraction followed by a concentration based on dialysis principle; and b) an immunoenzymatic detection using commercially available detection kits. ISO 19020:2017 is applicable to the screening of staphylococcal enterotoxins SEA to SEE in products intended for human consumption. Other staphylococcal enterotoxins such as types SEG, SEH, SEI, SER, SES and SET can also cause illness. Due to the lack of commercially available detection kits, ISO 19020:2017 is applicable only to types SEA to SEE, but may apply to other types of toxins, subject to validation of the method.