食物链微生物学 - 定量测定的测量不确定度评估

[中文适用范围]： 本文件规定了与食物链微生物学定量结果相关的测量不确定度（MU）的估计和表达的要求并提供了指导。 它适用于定量分析： ——供人类消费或喂养动物的产品； ——食品生产和食品处理领域的环境样品； ——初级生产阶段的样品。 定量分析通常通过使用菌落计数技术对微生物进行计数来进行。 本文件也普遍适用于其他定量分析，包括： ——最可能数（MPN）技术； ——仪器方法，如阻抗测定法、三磷酸腺苷（ATP）和流式细胞术； ——分子方法，例如基于定量聚合酶链式反应 (qPCR) 的方法。 本文件估计的不确定性不包括系统效应（偏差）。 [外文原描述]： This document specifies requirements and gives guidance for the estimation and expression of measurement uncertainty (MU) associated with quantitative results in microbiology of the food chain. It is applicable to the quantitative analysis of: — products intended for human consumption or the feeding of animals; — environmental samples in the area of food production and food handling; — samples at the stage of primary production. The quantitative analysis is typically carried out by enumeration of microorganisms using a colony-count technique. This document is also generally applicable to other quantitative analyses, including: — most probable number (MPN) techniques; — instrumental methods, such as impediometry, adenosine triphosphate (ATP) and flow cytometry; — molecular methods, such as methods based on quantitative polymerase chain reaction (qPCR). The uncertainty estimated by this document does not include systematic effects (bias).