水质.水和废水雌激素潜能的测定.第1部分：酵母雌激素筛选（酿酒酵母）

[中文适用范围]： 本文件规定了通过使用转基因酵母菌株酿酒酵母进行报告基因测定来测定水和废水的雌激素潜力的方法。 该报告基因测定基于人类雌激素受体α的激活。 本方法适用于： ——淡水； - 废水; ——水提取物和渗滤液； ——沉积物的洗出液（淡水）； ——孔隙水； ——单一物质或化学混合物的水溶液； ——饮用水。 根据国际实验室间试验的结果（见附件 F），该方法直接分析水样的定量限（LOQ）在 8 ng/l 至 15 ng/l 17β-雌二醇当量（EEQ）之间。 该测试的动态范围上限为 120 ng/l 至 160 ng/l 17β-雌二醇当量 (EEQ)。 显示雌激素效力高于此阈值的样品必须进行稀释才能进行有效的定量。 如果水样的雌激素潜力低于给定的 LOQ，则有必要对水样进行提取和预浓缩。 [外文原描述]： This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha. This method is applicable to: — fresh water; — waste water; — aqueous extracts and leachates; — eluates of sediments (fresh water); — pore water; — aqueous solutions of single substances or of chemical mixtures; — drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.