水质.水和废水雌激素潜能的测定.第2部分：酵母雌激素筛选

[中文适用范围]： 本文件规定了一种通过使用转基因酵母菌株 Arxula adeninivorans 进行报告基因测定来测定水和废水的雌激素潜力的方法。 该报告基因测定基于人类雌激素受体α的激活。 Arxula adeninivorans 是一种高度稳健、耐盐耐温的测试生物体，特别适合分析高盐度样品（电导率高达 70 mS/cm）。 测试微生物可以在氯化钠含量高达20%的培养基中培养。 本方法适用于： ——淡水； - 废水; ——海水； ——咸水； ——水提取物和渗滤液； ——沉积物的洗出液（淡水）； ——孔隙水； ——单一物质或化学混合物的水溶液； ——饮用水。 该方法直接分析水样的定量限 (LOQ) 为 1.5 ng/l 至 3 ng/l 17β-雌二醇当量 (EEQ)。 该测试的动态范围上限为 25 ng/l 至 40 ng/l 17β-雌二醇当量 (EEQ)。 显示雌激素效力高于此阈值的样品必须进行稀释才能进行有效的定量。 如果水样的雌激素潜力低于给定的 LOQ，则有必要对水样进行提取和预浓缩。 已经进行了一项国际实验室间试验来验证本文件。 结果总结于附录 F。 注：水样的提取和预浓缩是必要的。 1 [外文原描述]： This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with a genetically modified yeast strain Arxula adeninivorans. This reporter gene assay is based on the activation of the human estrogen receptor alpha. Arxula adeninivorans is a highly robust and salt- and temperature-tolerant test organism and is especially suitable for the analysis of samples with high salinity (conductivity up to 70 mS/cm). The test organism can be cultivated in medium with sodium chloride content up to 20 %. This method is applicable to: — fresh water; — waste water; — sea water; — brackish water; — aqueous extracts and leachates; — eluates of sediments (fresh water); — pore water; — aqueous solutions of single substances or of chemical mixtures; — drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 1,5 ng/l and 3 ng/l 17β-estradiol equivalents (EEQ). The upper threshold of the dynamic range for this test is between 25 ng/l and 40 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ. An international interlaboratory trial for the validation of this document has been carried out. The results are summarized in Annex F. NOTE Extraction and pre-concentration of water samples can prove necessary.