显微镜.照明特性的定义和测量.第3部分:非相干光源的入射光荧光显微镜

[中文适用范围]： 本文件规定了入射光荧光显微镜的照明亮度、时间稳定性和均匀性的测量程序。 当图像平面或中间图像平面适合由电子成像设备检测时，均匀性的测量仅在这些平面中定义。 本文件定义了如何测量照明亮度、时间稳定性和均匀性，以及如何将这些信息提供给用户。 注：范围有意限于电子成像设备和（中间）图像平面。 通过目镜进行的视觉观察将需要不同的测量程序，因此导致测量程序的描述含糊不清。 然而，本文档将对视觉观察的均匀性进行有用的估计，因为在这种情况下，使用目镜来观察中间图像平面（其在本文档的范围内）。 [外文原描述]： This document specifies procedures for the measurement of illumination brightness, temporal stability and uniformity for incident light fluorescence microscopy. The measurement for uniformity is defined in image planes or intermediate image planes only, when these planes are suitable for detection by electronic imaging devices. This document defines how illumination brightness, temporal stability and uniformity are measured, and how this information is provided to the user. NOTE The scope is intentionally limited to electronic imaging devices and (intermediate) image planes. The visual observation by means of eyepieces would require a different measurement procedure and hence result in ambiguities in the description of measurement procedures. Nevertheless, this document will give useful estimates for the uniformity with visual observation as in this case an eyepiece is used to observe an intermediate image plane (which is under the scope of this document).