标准摘要
[中文适用范围]： 本国际标准规定了通过流式细胞术定量乳制品中发酵剂中活性和/或总乳酸菌和益生菌菌株的标准化方法。 该方法还适用于乳制品中使用的益生菌和发酵乳制品，例如主要含有乳酸菌的酸奶。 本国际标准不适用于细菌的分类学分化。 由于其非特异性，该方法可以对样品中存在的本国际标准范围之外的其他细菌进行定量。 这可能会导致计数被高估。 应用此标准化方法之前样品中的最低细菌细胞浓度取决于各个方案中使用的稀释率。 通常每克或每毫升 106 个细胞被认为在最小范围内。 [外文原描述]： ISO 19344:2015 specifies a standardized method for the quantification of active and/or total lactic acid bacteria and probiotic strains in starter cultures used in dairy products by means of flow cytometry. The method is also applicable to probiotics used in dairy products and to fermented milk products such as yogurts containing primarily lactic acid bacteria. This International Standard does not apply to taxonomical differentiation of bacteria. Due to its non-specificity, the method may quantify other bacteria than those within the scope of this International Standard, when present in the sample. This may lead to overestimation of the counts. The minimum bacterial cell concentration in the sample before applying this standardized method depends on the dilution rates used in the individual protocols. Typically 10 6 cells per gram or ml are considered within the minimum range.
英文名称Milk and milk products — Starter cultures, probiotics and fermented products — Quantification of lactic acid bacteria by flow cytometry