采用高效液相色谱法(HPLC)测定尿素基肥料中的尿素含量

[中文适用范围]： 本文件规定了测定尿素基肥料中尿素含量的测试程序，包括尿素、尿素醛类[亚甲基尿素肥料、异丁烯二脲(IBDU)、巴豆叉二脲(CDU)]、尿素三嗪肥料、尿素硝酸铵(UAN)、硫磺和聚合物包覆尿素(SCU和PCU)，以及含尿素的复合肥料。该方法基于高效液相色谱法(HPLC)。 该方法是对AOAC官方方法2003.14的扩展，该方法在2003年对“水溶性尿素-甲醛肥料产品和尿素水溶液中尿素的测定”进行了协作研究。该方法于2004年发表在AOAC杂志上[4]，并于2003年获得首次行动，2008年获得最终行动。 该方法也适用于测定含尿素肥料中的双缩脲含量，结果于2014年发表在J. AOAC上[5]。该方法于2014年被国际标准化组织(ISO)作为委员会草案(ISO/CD 18643)采用，经过审查和环试验分析[6]。 注：除了EN 15705 [1]外，该HPLC方法还可用于分析上述肥料中的巴豆叉二脲(CDU)和异丁烯二脲(IBDU)含量。 [外文原描述]： ISO 19746:2017 specifies the test procedure for determining the urea content in urea-based fertilizers, including urea, urea aldehydes [methylene urea fertilizers, isobutylene diurea (IBDU), crotonylidene diurea (CDU)], urea triazone fertilizers, urea ammonium nitrate (UAN), sulfur- and polymer-coated urea (SCU and PCU), as well as compound fertilizers containing urea. The method is based on High Performance Liquid Chromatography (HPLC). The proposed method is an extension of the AOAC Official Method 2003.14 which was collaboratively studied for the "Determination of Urea in Water-Soluble Urea-Formaldehyde Fertilizer Products and in Aqueous Urea Solutions" in 2003. The method was published in the Journal of AOAC in 2004 [ 4 ] and was granted the First Action in 2003 and the Final Action in 2008. This method also applies to the determination of biuret content in urea containing fertilizer with the results published in the J. AOAC in 2014 [ 5 ] . This method was adopted by the International Organization for Standardization (ISO) as a Committee Draft (ISO/CD 18643) in 2014, and after review and the Ring Test Analyses [ 6 ] .