植物油 在线耦合高效液相色谱 气相色谱 火焰离子化检测器（HPLC-GC-FID）分析测定矿物油饱和烃（MOSH）和矿物油芳香烃（MOAH） 定量下限方法

[中文适用范围]： 本文件规定了使用在线耦合高效液相色谱-气相色谱-火焰离子化检测 (HPLC-GC-FID) 测定植物油脂中饱和烃和芳烃 (C10 至 C50) 的程序。[4][5][6] 本文件不适用于其他基质。该方法适用于矿物油饱和烃 (MOSH) 和/或矿物油芳烃 (MOAH) 的分析。根据实验室间研究的结果，该方法已被证明适用于 MOSH 质量浓度高于 3 mg/kg 和 MOAH 质量浓度高于 2 mg/kg 的情况。如果怀疑存在干扰，可以通过 GC⨯GC-MS 检查来验证 MOSH 和 MOAH 馏分的化石来源。附件 C 中提出了一种用于 MOAH 馏分环氧化的替代方法 (过甲酸环氧化)。该替代方法提供的结果与 8.6 中描述的 MOAH 馏分的乙醇环氧化相当。这种替代的环氧化方法已被证明对于 MOAH 馏分中干扰物较多的样品（如热带油）是有效的。[14] [外文原描述]： This document specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID). This document does not apply to other matrices. The method is applicable for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH). According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg. In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS. An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.6. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).