土壤质量.用微孔板中的比色基质测量土壤样品中的酶活性模式

[中文适用范围]： 本文件规定了同时（或不同时）测定多种水解酶活性（芳基酰胺酶、芳基硫酸酯酶、β-半乳糖苷酶、α-葡萄糖苷酶、β-葡萄糖苷酶、N-乙酰基-氨基葡萄糖苷酶、酸性、碱性和全局磷酸酶、脲酶）的方法。 土壤样品，使用比色底物。 土壤酶活性随季节变化，并取决于土壤化学、物理和生物特性。 该方法可用于检测受污染土壤中有毒物质或其他人为因素对对照土壤中土壤酶活性的有害影响，也可用于测试化学品。 [外文原描述]： This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, β -galactosidase, α -glucosidase, β -glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activities derived from toxic substances or other anthropogenic agents in contaminated soils against a control soil, or to test chemicals.