纺织品.一些动物毛发纤维的定性和定量蛋白质组学分析.第1部分：使用蛋白质还原的LC-ESI-MS的肽检测

[中文适用范围]： 本标准规定了通过显微镜初步筛选、蛋白质提取、酶消化和配备电喷雾电离的液相色谱-质谱联用仪检测特定肽来测定纺织品中羊毛、羊绒、牦牛纤维及其混纺物含量的定性和定量测试方法来源（LCI-ESI-MC）。 该方法可应用于各个工艺阶段（即从原材料到服装）的相关纺织产品，且成分分布均匀。 它可应用于含有羊毛、羊绒或牦牛纤维及其混纺的不同类型的纺织材料（例如短纤、毛条、纱线和织物）。 该方法基于通过光学显微镜根据其形态对共混物中的所有纤维进行初步鉴定。 然后用硫脲/尿素/二硫苏糖醇 (DTT) 溶液提取蛋白质。 通过胰蛋白酶对从纤维中提取的蛋白质进行酶消化。 通过 LC-MS 进行特定标记物的分析并计算组成百分比。 该方法通过将其结果与使用 ISO 1833 系列和/或 ISO 17751 系列获得的结果相结合，适用于含有除羊毛、羊绒和牦牛之外的其他种类纤维的样品。 如果存在相同动物物种的纤维（例如羊绒和马海毛的混合物），则本文件不适用；在这种情况下，可以使用显微镜分析进行定量分析（例如 [外文原描述]： ISO 20418-1:2018 specifies a qualitative and quantitative testing method to determine the content of wool, cashmere, yak fibres and their blends in textiles by microscope preliminary screening, protein extraction, enzymatic digestion and specific peptides detection using a liquid chromatography-mass spectrometer equipped with electrospray ionization source (LCI-ESI-MC). This method can be applied to relevant textile products at each process stage (i.e. from raw material to garment) with a homogeneous distribution of the components. It can be applied to different types of textile materials (e.g. staples, tops, yarns and fabrics) that contain wool, cashmere or yak fibres and their blends. The method is based on a preliminary identification of all fibres in the blend on the basis of their morphology, by light microscopy. The proteins are then extracted by a thiourea/urea/dithiothreitol (DTT) solution. An enzymatic digestion by trypsin of the protein extracted from the fibres is carried out. Analysis of the specific markers is performed by LC-MS and the percent composition is calculated. This method is applicable to samples containing other kinds of fibres than wool, cashmere and yak, by combining its results with the results obtained using the ISO 1833 series and/or the ISO 17751 series. ISO 20418-1:2018 does not apply if fibres of the same animal species are present (e.g. blends of cashmere and mohair); in this case, the quantitative analysis can be performed using microscopic analysis (e.g. ISO 17751 series).