标准摘要
[中文适用范围]： 本文件规定了通过分子方法（例如聚合酶链反应（PCR））检测核酸序列（DNA）的性能特征的最低要求，包括不同的PCR后检测方法、实时PCR、单次和/或多次PCR基于探针的检测技术以及这些方法的组合。 该文件适用于食品中高等和低等分类群动物物种DNA的检测、鉴定和定量，以及适用方法的验证。 适用于哺乳动物、鸟类、爬行动物、两栖动物、鱼类、软体动物、甲壳动物和昆虫。 附录 A 中列出了每种情况的典型示例。 [外文原描述]： This document specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA) by molecular methods, such as the polymerase chain reaction (PCR), including different post-PCR detection methods, real-time PCR, single and/or multiple probe-based detection techniques as well as the combination of such methods. The document is applicable to the detection, identification and quantification of DNA from animal species of higher and lower taxonomic groups in foodstuffs, and the validation of applicable methods. It is applicable to mammals, birds, reptiles, amphibians, fishes, molluscs, crustaceans and insects. Typical examples for each are listed in Annex A.
英文名称Molecular biomarker analysis — Methods of analysis for the detection and identification of animal species in foods and food products (nucleic acid-based methods) — General requirements and definitions