植物脂肪和油 免疫亲和柱净化和高效液相色谱法测定黄曲霉毒素B1、B2、G1和G2

[中文适用范围]： 本文件规定了使用免疫亲和柱净化和高效液相色谱法（HPLC）结合柱后衍生化测定植物油脂中黄曲霉素B₁、B₂、G₁和G₂的方法。适用的植物油脂包括花生油、芝麻油、橄榄油、玉米油、太阳花油、菜籽油和椰子油。本方法规定了黄曲霉素B₁、B₂、G₁和G₂以及它们总和的定量限分别为1 μg/kg、0.25 μg/kg、0.5 μg/kg、0.25 μg/kg和1 μg/kg。通过以下浓度范围对方法进行了验证：-黄曲霉素B₁=1 μg/kg至20 μg/kg；-总黄曲霉素=2 μg/kg至52 μg/kg。 [外文原描述]： This document specifies a method for the determination of the aflatoxins B 1 , B 2 , G 1 and G 2 in vegetable fats and oils, including peanut oil, sesame oil, olive oil, corn oil, sunflower oil, rapeseed oil and coconut oil, using immunoaffinity column clean-up and high-performance liquid chromatography with post-column derivatization. The limits of quantification for the aflatoxins B 1 , B 2 , G 1 and G 2 , and for the sum of aflatoxins B 1 , B 2 , G 1 and G 2 , are 1 μg/kg, 0,25 μg/kg, 0,5 μg/kg, 0,25 μg/kg and 1 μg/kg, respectively. The validation was carried out over the following concentration ranges: — aflatoxin B 1 = 1 μg/kg to 20 μg/kg; — total aflatoxins = 2 μg/kg to 52 μg/kg.