标准摘要
[中文适用范围]： 本文件规定了使用虹鳟鱼（Oncorhynchus mykiss）鳃永久细胞系 RTgill-W1 测定鱼类急性毒性的方法。 将 24 孔组织培养板中汇合单层的细胞暴露于水样（例如地表水或不同种类的废水）或化学品中 24 小时，然后根据荧光细胞活力指示剂染料评估细胞活力（参见4.1）。 然后，数据表示为未暴露对照的百分比，并根据细胞活力的百分比与响应曲线中的流出物或化学浓度的百分比进行量化（参见第 9 条）。 [外文原描述]： This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).
英文名称Water quality — Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)