化妆品.微生物学.大肠杆菌的检测

[中文适用范围]： 本国际标准提供了在化妆品产品中检测和鉴定特定微生物大肠杆菌的一般指南。本标准中考虑的微生物可能因国家实践或法规的不同而有所差异。为确保产品质量和消费者安全，建议进行适当的微生物风险分析，以确定本标准适用的化妆品类型。低微生物风险产品（参见ISO 29621）包括那些具有低水分活度、醇水产品、极端pH值等。本标准描述的方法基于在非选择性液体培养基（富集肉汤）中检测大肠杆菌，随后在选择性琼脂培养基上分离。根据所需的检测水平，其他方法可能适用。 [外文原描述]： ISO 21150:2015 gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc. The method described in this International Standard is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate, depending on the level of detection required. NOTE For the detection of Escherichia coli, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits). Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) can be substituted for the test presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.