标准摘要
[中文适用范围]： ISO 21427 的这一部分规定了使用哺乳动物体外试验测定水和废水遗传毒性的方法，该试验检测水溶性物质对中国仓鼠 V79 细胞染色体或有丝分裂器造成的损伤。 微核测试可以识别引起细胞遗传学损伤的物质，从而导致含有滞后染色体片段和/或整个染色体的微核的形成。 该测定基于在有和没有代谢激活的情况下孵育后微核细胞频率的增加。 [外文原描述]： ISO 21427-2:2006 specifies a method for the determination of genotoxicity of water and waste water using a mammalian in vitro test which detects damage, induced by water-soluble substances, to the chromosomes or the mitotic apparatus of V79 cells from the Chinese hamster. The micronucleus test allows the identification of substances that cause cytogenetic damage which results in the formation of micronuclei containing lagging chromosome fragments and/or whole chromosomes. The assay is based on the increase in the frequency of micronucleated cells after incubation with and without metabolic activation.
英文名称Water quality — Evaluation of genotoxicity by measurement of the induction of micronuclei — Part 2: Mixed population method using the cell line V79