标准摘要
[中文适用范围]: 本国际标准提供了使用聚合酶链反应(PCR)检测食品中转基因生物(GMO)的定量方法的总体框架。 它定义了 DNA 靶序列特定扩增的一般要求,以便量化相对转基因生物 DNA 含量并确认扩增 DNA 序列的身份。 本国际标准规定的指南、最低要求和性能标准旨在确保在不同实验室获得可比较、准确和可重复的结果。 该国际标准是针对食品基质制定的,但也适用于其他基质,例如饲料和环境中的植物样品。 附件 A 至 D 中提供了方法的具体示例。 [外文原描述]: ISO 21570:2005 provides the overall framework of quantitative methods for the detection of genetically modified organisms (GMO) in foodstuffs, using the polymerase chain reaction (PCR). It defines general requirements for the specific amplification of DNA target sequences in order to quantify the relative GMO-derived DNA content and to confirm the identity of the amplified DNA sequence. Guidelines, minimum requirements and performance criteria laid down in ISO 21570:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories. ISO 21570:2005 has been established for food matrices, but is also applicable to other matrices, e.g. feed and plant samples from the environment.
英文名称Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Quantitative nucleic acid based methods