土质.生物方法.高等植物的慢性毒性

[中文适用范围]： 本国际标准描述了在受控条件下测定土壤对高等植物生长和繁殖能力的抑制的方法。 推荐两种物种：芜菁油菜 (Brassica rapa CrGC syn. Rbr) 和燕麦 (Avena sativa) 的快速循环变种。 测试持续时间应足以包括证明测试植物繁殖能力的长期终点。 通过使用天然测试土壤，例如来自污染场地或修复的土壤，并通过将这些土壤中测试植物的发育与参考或标准对照土壤进行比较，该测试可用于评估土壤质量，尤其是土壤的功能：植物的栖息地。 附件 A 描述了允许使用慢性植物测定来测试土壤中的化学物质的修改。 通过在标准对照土壤中制备测试物质的一系列稀释液，可以测量相同的终点来评估化学品的慢性毒性。 该方法不适用于挥发性物质，即H（亨利常数）或空气/水分配系数大于1，或25℃时蒸气压超过0.013 3 Pa的物质。 [外文原描述]： ISO 22030:2005 describes a method for determining the inhibition of the growth and reproductive capability of higher plants by soils under controlled conditions. Two species are recommended: a rapid-cycling variant of turnip rape ( Brassica rapa CrGC syn. Rbr) and oat ( Avena sativa ). The duration of test should be sufficient to include chronic endpoints that demonstrate the reproductive capability of the test plants. By using natural test soils, e.g. from contaminated sites or remediated soils, and by comparing the development of the test plants in these soils with reference or standard control soils, the test is applicable to assess soil quality, especially the function of the soil as a habitat for plants. This method can be modified to allow use of the chronic plant assay for the testing of chemicals incorporated into soil. By preparing a dilution series of a test substance in standard control soils, the same endpoints can be measured to assess the chronic toxicity of chemicals. This method is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.