化妆品.微生物学.绿脓杆菌的测定

[中文适用范围]： 本国际标准给出了化妆品中特定微生物铜绿假单胞菌检测和鉴定的一般指南。 根据国家惯例或法规，本国际标准中规定的微生物可能因国家而异。 为了确保产品质量和消费者安全，建议进行适当的微生物风险分析，以确定本国际标准适用的化妆品类型。 被认为存在低微生物风险（参见 ISO 29621）的产品包括低水分活度、水醇产品、极端 pH 值等。 本国际标准中描述的方法基于非接触环境中铜绿假单胞菌的检测。 选择性液体培养基（增菌肉汤），然后在选择性琼脂培养基上进行分离。 其他方法可能也适用，具体取决于所需的检测水平。 注：为了检测铜绿假单胞菌，可以在非选择性培养基上进行继代培养，然后进行适当的识别步骤（例如使用识别试剂盒）。 由于该应用领域内的化妆品种类繁多，因此该方法可能并不适合某些产品（例如某些与水不混溶的产品）的每个细节。 其他国际标准 (ISO 18415) 可能也适用。 其他方法（例如自动方法）可以替代此处提出的测试，只要它们的等效性已被证明或该方法已被证明是合适的。 [外文原描述]： ISO 22717:2015 gives general guidelines for the detection and identification of the specified microorganism Pseudomonas aeruginosa in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc. The method described in this International Standard is based on the detection of Pseudomonas aeruginosa in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate, depending on the level of detection required. NOTE For the detection of Pseudomonas aeruginosa, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits). Because of the large variety of cosmetic products within this field of application, this method may not be appropriate in every detail for some products (e.g. certain water immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.