动植物油脂.在线配体受体复合色谱和荧光检测法的高效液相色谱进行多环芳烃的测定

[中文适用范围]： 本国际标准规定了一种高效液相色谱（HPLC）程序，用于测定食用脂肪和油中的多环芳烃（PAHs）。该方法已针对椰子油（CN）、橄榄油（OV）、葵花籽油（SF）和大豆油（BO）进行了验证，并可能适用于其他油类，具体取决于适当参数的确定。PAHs的最低定量水平为0.1 µg/kg。尚未确定能够与基线噪声区分开来的每种PAH的最低可能量。该方法的验证浓度范围为每种PAH的0.1 µg/kg至3.5 µg/kg。对于含有（轻）PAH含量> 3.5 µg/kg的样品，可稀释至验证范围内。也可以调整校准曲线的范围。然而，超过3.5 µg/kg的范围尚未验证。本方法可测定的PAHs包括：蒽、菲、荧蒽、芘、䓛、苯并[a]蒽、苯并[e]芘、苯并[a]芘、苝、苯并[ghi]芘、苯并[a]荧蒽、苯并[b]荧蒽、苯并[k]荧蒽、屈、苯并[a]荧蒽、苯并[b]荧蒽、苯并[k]荧蒽。 [外文原描述]： ISO 22959:2009 specifies a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils. The method has been validated for coconut, olive, sunflower, and soybean oil, and is possibly applicable to other oils, dependent on the determination of appropriate parameters. The lowest level of quantification for the PAHs is 0,1 µg/kg. The lowest possible amount of each PAH which can be distinguished from the baseline noise has not been determined. The validated concentration range of the method is 0,1 µg/kg to 3,5 µg/kg for each individual PAH. For samples containing (light) PAH contents > 3,5 µg/kg, dilution to bring the contents into the validated range is possible. It is also possible to adjust the range of the calibration curves. However, ranges exceeding 3,5 µg/kg have not been validated. PAHs which can be determined by this method are: anthracene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[ a ]anthracene, benzo[ e ]pyrene, benzo[ a ]pyrene, perylene, benzo[ ghi ]perylene, anthanthrene, dibenzo[ a , h ]anthracene, coronene, indeno[1,2,3- cd ]pyrene, benzo[ a ]fluoranthene, benzo[ b ]fluoranthene, benzo[ k ]fluoranthene.