食物链的微生物学.鱼类和渔业产品中异足目L3幼虫的检测方法.第2部分：人工消化法

[中文适用范围]： 本文件规定了一种检测海洋鱼类和溯河产卵鱼类中常见的异尖线虫科 L3 幼虫的方法。 该方法适用于鲜鱼和/或冷冻鱼，以及轻度加工的鱼制品，例如腌制、腌制或熏制的鱼制品。 它也适用于内脏器官，作为目视检查方案的验证方法。 人工消化方法[4][5][6]适用于通过估计鱼肌肉组织中寄生虫的数量来量化寄生虫感染，并且当应用于新鲜鱼或轻度加工的鱼制品（加工前从未冷冻）时，确定Anisakidae L3 的生存能力，可以存在。 该方法不适用于确定检测到的寄生虫的种类或基因型。 最终鉴定是通过形态学和/或分子方法进行的。 [外文原描述]： This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme. The artificial digestion method [4][5][6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present. This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.