水质 多氯二苯并-对-二恶英和多氯联苯的选定同系物的检测 使用流式免疫传感器技术的方法

[中文适用范围]： 本文件规定了使用流动免疫传感器检测水和废水中选定的多氯二苯并二恶英 (PCDD) 和多氯联苯 (PCB) 同系物的方法和原理。 流式免疫传感器利用 2,3,7,8-四氯二苯并-对二恶英 (2,3,7,8-TCDD) 和 3,3',4,4',5-五氯联苯 (3,3' ,4,4',5-PeCB)，在 PCDD 和 PCB 的同系物中具有最高的毒性当量因子 (TEF) 值。 该方法适用于及时监测水和废水中选定的 2,3,7,8-TCDD 和 3,3',4,4',5-五氯苯同系物，以便优先进行后续确证测定。 本文件规定了流式免疫传感器中的采样、提取、净化、测量、数据处理和测量结果验证的实用方法和程序。 结合使用自动化仪器进行提取、净化和流动免疫传感可以减少时间消耗和劳动强度，同时提供可重复的精确数据。 该方法在 20 ℃ 时可提供 2,3,7,8-TCDD 和 3,3',4,4',5-PeCB 的定量下限 (LOQ) 分别为 28 pg/l 和 152 pg/l。 % 或更少的系数变异 (CV)，具体取决于采样、提取、净化和测量条件。 [外文原描述]： This document specifies methods and principles for detection of selected congeners of polychlorinated dibenzo- p -dioxins (PCDDs) and polychlorinated biphenyls (PCBs) in water and wastewater using a flow immunosensor. The flow immunosensor utilizes antibodies specific to 2,3,7,8- tetrachlorodibenzo- p -dioxin (2,3,7,8-TCDD) and 3,3’,4,4’,5- pentachlorobiphenyl (3,3’,4,4’,5-PeCB), which have the highest toxic equivalent factor (TEF) value among the congeners of each of PCDDs and PCBs. The method is applicable to timely monitoring of selected congeners of 2,3,7,8-TCDD and 3,3’,4,4’,5-PeCB in water and wastewater to prioritize those for subsequent confirmatory determination. This document specifies practical methods and procedures for sampling, extraction, clean-up, measurement in a flow immunosensor, data processing and validation of measurement results. The combined use of automated instruments for extraction, clean-up, and flow immunosensing can reduce time-consumption and labour-intensity, while providing reproducible precise data. This method can provide the lower limit of quantification (LOQ) for 2,3,7,8-TCDD and 3,3’,4,4’,5-PeCB of 28 pg/l and 152 pg/l, respectively at 20 % or less of coefficient variation (CV) depending on sampling, extraction, clean-up and measurement conditions.