土壤质量暴露于土壤中污染物的杀螨（Oppia nitens）繁殖抑制试验

[中文适用范围]： 本文件规定了评估土壤栖息地功能并确定土壤污染物和单个化学物质对 Oribatid 螨 Oppia nitens 通过皮肤和食物吸收繁殖的影响的方法之一。 这种长期（28天）测试适用于质量未知的土壤和土壤材料（例如，污染场地、改良土壤、修复后的土壤、农业或其他受关注的场地和废料）。 该方法无意取代蚯蚓或跳虫测试，因为它代表另一个分类群（=螨；即蛛形纲动物），也无意取代捕食性螨测试，因为该物种代表不同的营养水平和生态位。 使用标准土壤，最好是指定的人造土壤基质来评估物质的影响。 对于受污染的土壤，在测试土壤和对照土壤中确定影响。 根据研究目的，对照和稀释基质（污染土壤的稀释系列）应为与待测土壤样品性质相似的未污染土壤（参考土壤）或标准土壤（例如人造土壤） 。 提供了有关如何使用此方法在温和条件下测试物质的信息。 本文件不适用于空气/土壤分配系数大于1的物质，或25℃时蒸气压超过300Pa的物质。 注：在测试期间不能保证测试物质的稳定性。 测试方法中没有规定监测受试物质的持久性。 [外文原描述]： This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and individual chemical substances on the reproduction of the oribatid mite Oppia nitens by dermal and alimentary uptake. This chronic (28-day) test is applicable to soils and soil materials of unknown quality (e.g., contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials). This method is not intended to replace the earthworm or Collembola tests since it represents another taxonomic group (= mites; i.e., arachnids), nor the predatory mite test since this species represents a different trophic level and ecological niche. Effects of substances are assessed using standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil with similar properties to the soil sample to be tested (reference soil) or a standard soil (e.g., artificial soil). Information is provided on how to use this method for testing substances under temperate conditions. This document is not applicable to substances for which the air/soil partition coefficient is greater than 1, or to substances with vapour pressure exceeding 300 Pa at 25 °C. NOTE The stability of the test substance cannot be assured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.