标准摘要
[中文适用范围]： 本文件规定了生成和分析从食物链获得的细菌的全基因组测序 (WGS) 数据的最低要求。 该过程可包括以下阶段： a) 细菌培养物的处理； b) 纯基因组 DNA 分离； c) 文库制备、测序以及原始 DNA 序列读取质量和存储的评估； d) 用于确定遗传相关性、遗传内容和预测表型的生物信息学分析，以及生物信息学管道验证； e) 元数据捕获和序列存储库沉积； f) 验证端到端 WGS 工作流程（适合预期应用）。 本文件适用于从以下来源分离出的细菌： ——供人类食用的产品； ——用于动物饲料的产品； ——来自食品和饲料处理和生产区域的环境样品； ——初级生产阶段的样品。 [外文原描述]： This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages: a) handling of bacterial cultures; b) axenic genomic DNA isolation; c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage; d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation; e) metadata capture and sequence repository deposition; f) validation of the end-to-end WGS workflow (fit for purpose for intended application). This document is applicable to bacteria isolated from: — products intended for human consumption; — products intended for animal feed; — environmental samples from food and feed handling and production areas; — samples from the primary production stage.
英文名称Microbiology of the food chain — Whole genome sequencing for typing and genomic characterization of bacteria — General requirements and guidance