婴儿配方奶粉和成人营养品 反相超高效液相色谱法测定β-胡萝卜素、番茄红素和叶黄素

[中文适用范围]： 本文件规定了使用反相超高性能定量测定固体（即粉末）或液体（即即食液体和液体浓缩物）形式的婴儿配方奶粉和成人营养品中β-胡萝卜素和番茄红素的方法液相色谱（RP-UHPLC）和紫外-可见检测。 番茄红素的应用范围为 1 μg/100 g 至 1500 μg/100 g，β-胡萝卜素的应用范围为 1 μg/100 g 至 2250 μg/100 g。 根据单一实验室验证，每种类胡萝卜素的检测限 (LOD) 为 0.1 μg/100 g，定量限 (LOQ) 为 0.3 μg/100 g。 该方法不适用于含有可测量水平的 β-apo-8'-胡萝卜素的材料。 重现性数据满足参考文献[8]和[10]中给出的要求。 附录C规定了叶黄素的测定。 重现性数据不满足参考文献[9]给出的要求。 [外文原描述]： This document specifies a method for the quantitative determination of β-carotene and lycopene in infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) and UV-visible detection. The application range runs from 1 μg/100 g to 1 500 μg/100 g for lycopene and from 1 μg/100 g to 2 250 μg/100 g for β-carotene. Based on the single-laboratory validation, the limit of detection (LOD) was 0,1 μg/100 g and the limit of quantification (LOQ) was 0,3 μg/100 g for each carotenoid. The method does not apply to materials that contain measurable levels of β-apo-8′-carotenal. The reproducibility data meets the requirements given in References [8] and [10]. Annex C specifies the determination of lutein. The reproducibility data does not meet the requirements given in Reference [9].