标准摘要
[中文适用范围]： 本文件规定了从陆地现场土壤中采样和处理自由生活的线虫的方法，作为将其用作生物指标（例如评估土壤作为有机体栖息地的质量）的前提条件。本文件适用于所有发生线虫的陆地生境。场研究的一般采样设计在 ISO 18400-101 中指定。由于样品基质（例如水柱）的不同，本文件不适用于水生线虫。针对其他土壤生物群的方法，如蚯蚓、弹尾目、enchytraeids 或大型无脊椎动物，请参见 ISO 23611-1、ISO 23611-2、ISO 23611-3 和 ISO 23611-5。本文件不包括采样地点的土壤特征，这在采集土壤生物时强烈推荐进行。ISO 10390、ISO 10694、ISO 11272、ISO 11274、ISO 11277、ISO 11461 和 ISO 11465 包含适合用于测量 pH 值、颗粒大小分布、C/N 比率、有机碳含量和持水能力的程序。 [外文原描述]： This document specifies a method for sampling and handling free-living nematodes from terrestrial field soils as a prerequisite for using them as bio-indicators (e.g. to assess the quality of a soil as a habitat for organisms). This document applies to all terrestrial biotopes in which nematodes occur. The sampling design of field studies in general is specified in ISO 18400-101. This document is not applicable to aquatic nematodes because of differences in the sample matrix (e.g. water column). Methods for some other soil organism groups such as earthworms, collembolans enchytraeids or macro-invertebrates are covered in ISO 23611-1, ISO 23611-2, ISO 23611-3 and ISO 23611-5. This document does not cover the pedological characterization of the site which is highly recommendable when sampling soil invertebrates. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 and ISO 11465 include suitable procedures for measuring pH, particle size distribution, C/N ratio, organic carbon content and water-holding capacity.
英文名称Soil quality — Sampling of soil invertebrates — Part 4: Sampling, extraction and identification of soil-inhabiting nematodes