标准摘要
[中文适用范围]： 完整描述 本文件描述了核苷酸序列数据库评估和验证的实用程序。本文件描述了核苷酸序列数据库验证的最低要求。本文件仅适用于由核苷酸序列条目组成的数据库。本文件不适用于对整个数据库质量（包括每个数据条目的质量）的一般评估。示例：使用经过验证的数据库来确认代表性序列特异性，包括通过常规聚合酶链式反应 (PCR)、定量聚合酶链式反应 (qPCR)、数字聚合酶链式反应 (dPCR) 和微阵列技术对目标核酸进行鉴定和定量的引物或探针。 [外文原描述]： This document describes a practical procedure for nucleotide sequence database evaluation and validation. This document describes minimum requirements for the validation of a nucleotide sequence database. This document is applicable only for databases consisting of entries of nucleotide sequences. This document is not applicable to the general evaluation of the entire database quality including the quality of each data entry. EXAMPLE The use of the validated database is for confirming a representative sequence specificity including primers or probes for qualification and quantification of target nucleic acids by conventional polymerase chain reaction (PCR), quantitative polymerase chain reaction (qPCR), digital polymerase chain reaction (dPCR) and microarray technologies.
英文名称Biotechnology — Validation of database used for nucleotide sequence evaluation