标准摘要
[中文适用范围]： 本文件规定了用于评估纳米粒子（NPs）悬浮在细胞培养基中，在近紫外线（UVA）照射下（315 nm到400 nm）测量相对细胞活力下降的方法。该测量旨在通过将NP的光毒性与阳性对照氯promazine进行比较，评估NP的急性光毒性的潜力。本方法不用于预测纳米材料和光的其他联合效应，例如基因毒性、光过敏或光诱变性。该方法还适用于NP聚集体和聚集物。 [外文原描述]： This document specifies a procedure to evaluate acute phototoxicity of nanoparticles (NPs), suspended in cell culture media, by measuring the relative reduction in cellular viability under near ultraviolet (UVA) exposure (315 nm to 400 nm). The measurement is intended to assess the potential for acute phototoxicity of NPs by comparing NP photoactivity in vitro to a positive control chlorpromazine. It is not designed to predict other joint effects of nanomaterials and light, such as genotoxicity, photo-allergy or photo-mutagenicity. The method is also applicable to NP aggregates and agglomerates.
英文名称Nanotechnologies — In vitro acute nanoparticle phototoxicity assay