动植物油脂酚类抗氧化剂的高效液相色谱分析

[中文适用范围]： 本文件规定了高效液相色谱法（HPLC）分析酚类抗氧化剂的方法。适用于定量检测添加到动物和植物脂肪、油和人造奶油中的合成酚类化合物作为抗氧化剂的情况，浓度通常为油中正常添加：-丙基没食子酸酯（PG）；-辛基没食子酸酯（OG）；-十二烷基没食子酸酯（也称为‘月桂酰没食子酸酯（LG）’）；-2,4,5-三羟基丁基对苯二酚（THBP）；-叔丁基对氢醌（TBHQ）；- nordihydroguaiaretic 酸（NDGA）；- 2-和3-叔丁基-4-羟基茴香醚（BHA）；-2,6-di-tert-butyl-4-(hydroxymethyl)phenol（BHT Alcohol 或 Ionox-100）；-2,6-di-tert-butyl-4-hydroxytoluene（BHT）。附录 B 提供了确定没有抗氧化剂或在分析限制内的微量抗氧化剂的方法。关于芥子油中天然存在的物质 canolol 干扰分析的问题，参见附录 C。 [外文原描述]： This document specifies a method for the analysis of phenolic antioxidants by high-performance liquid chromatography (HPLC). It is applicable to quantifying the following synthetic phenolic compounds added to animal and vegetable fats, oils and shortenings as antioxidants, at concentrations normally added to oils: — propyl gallate (PG); — octyl gallate (OG); — dodecyl gallate (also called “lauryl gallate (LG)”); — 2,4,5-trihydroxybutyrophenone (THBP); — tert-butylhydroquinone (TBHQ); — nordihydroguaiaretic acid (NDGA); — 2- and 3-tert-butyl-4-hydroxyanisole (BHA); — 2,6-di-tert-butyl-4-(hydroxymethyl)phenol (BHT Alcohol or Ionox-100); — 2,6-di-tert-butyl-4-hydroxytoluene (BHT). A method for determining the absence of an antioxidant, or the maximum trace amount, within the limits of the analysis, is given in Annex B. The issue of canolol, a naturally occurring substance in rapeseed, interfering with the analysis is addressed in Annex C.